Tag Archives | Steps in Gene Cloning

In gene cloning, the major steps are cut­ting and joining lengths of DNA using re­striction endonuclease and ligase. Several steps are involved in gene cloning. 1. Preparation of cloning vector: Before cloning the fragment of interest, the plasmid (cloning vector) is to be restric­tion digested. Plasmids are small extra chromosomal, self-replicating, circular double stranded DNA […]