Upload NowIn this article we will discuss about the principle, requirements and procedure for silver staining of proteins. Polypeptides even upto 0.1 µg present on gel can be visualised by staining with amido black 10B dye or Coomassie brilliant blue R250. Silver staining is useful since some proteins occur in very small quantities and are difficult […]
Silver Staining of Proteins | Molecular Biology
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Separating Micro-Weights of Proteins: PAGE | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Polyacrylamide Gel Electrophoresis (PAGE). Micro-weights of proteins can be separated and characterised rapidly and relatively sensitive, using PAGE. Buffer gels (non-denaturing) as well as Sodium Dodecyl Sulphate (SDS) containing denaturing gels are used. Separation in buffer gels depends on both the charge […]
Extraction and Separation of Storage Proteins | Plant Biotechnology
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In this article we will discuss about the requirements, reagents and procedure for extraction and separation of storage proteins. Requirements: 1. Legume seeds/serial grains. 2. Grinder. 3. Refrigerator. 4. Centrifuge. 5. Spectronic 20. 6. Magnetic stirrer. 7. TRIS base. 8. NaCl. 9. Anhydrous Na2SO4. 10. NaOH. 11. Na-K-tartarate. 12. Na2CO3. 13. CuSO4.7H2O. 14. Folin-Ciocalteau reagent. […]