Upload NowHybridisation reactions are based on the ability of denatured DNA to anneal to a complementary strand present in a nearby environment at a temperature below that of theirs. The two important features of hybridisation are: (1) These reactions are specific i.e. the probe will only bind to targets with complementary sequence. (2) These reactions will […]
Southern Hybridisation | Molecular Biology
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Estimation of Reducing Sugar: 4 Methods | Plant Biotechnology
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The following points highlight the top four methods used for estimation of reducing sugar. The methods are: 1. Nelson—Somogyi Method 2. Folin Wu Method 3. Dinitrosalicylic Acid Method 4. Glucose Oxidase Method. 1. Nelson—Somogyi Method: Sugars arising out of the presence of an aldehyde or ketogroup with reducing property like glucose, maltose, lactose and galactose […]
Estimation of Proteins: 2 Methods | Plant Biotechnology
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The following points highlight the top two methods used for the estimation of proteins. The methods are: 1. Lowry’s Method 2. Bradford’s (1976) Method. 1. Lowry’s Method: This method gives a moderately constant value of proteins. Hence protein content of enzymes is usually determined by this method. Principle: Amino acids tryptophan and tyrosine present in […]