Upload NowHybridisation reactions are based on the ability of denatured DNA to anneal to a complementary strand present in a nearby environment at a temperature below that of theirs. The two important features of hybridisation are: (1) These reactions are specific i.e. the probe will only bind to targets with complementary sequence. (2) These reactions will […]
Southern Hybridisation | Molecular Biology
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Estimation of Cellulose | Plant Biotechnology
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In this article we will discuss about the principle, requirements and procedure for estimation of cellulose. Cellulose is the major component in plants. It is composed of glucose molecules joined together in the form of repeating units of cellobiose with several cross linkages. Principle: With acetic/nitric reagents cellulose undergoes acetolysis forming acetylated cellod-extrins. These cellodextrins […]
Extraction and Separation of Storage Proteins | Plant Biotechnology
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In this article we will discuss about the requirements, reagents and procedure for extraction and separation of storage proteins. Requirements: 1. Legume seeds/serial grains. 2. Grinder. 3. Refrigerator. 4. Centrifuge. 5. Spectronic 20. 6. Magnetic stirrer. 7. TRIS base. 8. NaCl. 9. Anhydrous Na2SO4. 10. NaOH. 11. Na-K-tartarate. 12. Na2CO3. 13. CuSO4.7H2O. 14. Folin-Ciocalteau reagent. […]