Upload NowHybridisation reactions are based on the ability of denatured DNA to anneal to a complementary strand present in a nearby environment at a temperature below that of theirs. The two important features of hybridisation are: (1) These reactions are specific i.e. the probe will only bind to targets with complementary sequence. (2) These reactions will […]
Southern Hybridisation | Molecular Biology
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Extraction and Separation of Storage Proteins | Plant Biotechnology
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In this article we will discuss about the requirements, reagents and procedure for extraction and separation of storage proteins. Requirements: 1. Legume seeds/serial grains. 2. Grinder. 3. Refrigerator. 4. Centrifuge. 5. Spectronic 20. 6. Magnetic stirrer. 7. TRIS base. 8. NaCl. 9. Anhydrous Na2SO4. 10. NaOH. 11. Na-K-tartarate. 12. Na2CO3. 13. CuSO4.7H2O. 14. Folin-Ciocalteau reagent. […]
Estimation of Reducing Sugar: 4 Methods | Plant Biotechnology
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The following points highlight the top four methods used for estimation of reducing sugar. The methods are: 1. Nelson—Somogyi Method 2. Folin Wu Method 3. Dinitrosalicylic Acid Method 4. Glucose Oxidase Method. 1. Nelson—Somogyi Method: Sugars arising out of the presence of an aldehyde or ketogroup with reducing property like glucose, maltose, lactose and galactose […]