Upload NowIn this article we will discuss about the isolation and collection of microorganisms present on the leaf. Isolation of Microorganisms on Leaf: Leaf Impression Method: Principle: By this method almost all species of organisms can be brought into culture. Requirements: 1. Agar plates—Bacterial, fungal and Actinomycete media. 2. Undamaged healthy mature leaves. 3. Graph paper. […]
Microorganisms Present on Leaf | Biotechnology
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Isolation of Microorganisms from Seed | Biotechnology
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The following points highlight the top four methods used for isolation of microorganisms present on the seed. The methods are: 1. Seed Washings 2. Dry Seed Agar Plates 3. Blotter Technique 4. Collection of Seed Leachates. Method # 1. Seed Washings: Principle: Maximum number of organisms can be brought into culture by inoculating the washings […]
Plant Tissue Culture | Plant Biotechnology
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In this article we will discuss about:- 1. Meaning of Plant Tissue Culture 2. Culture Media 3. Applications of Tissue Culture 4. Callus Culture 5. Organogenesis 6. Embryogenesis 7. Culture of Zygotic Embryo 8. Microspore Culture 9. Isolation of Plant Protoplast 10. Protoplast Fusion 11. Cybrids 12. Meristem Culture 13. Agrobacterium Mediated Transformation System and […]