Upload NowIn this article we will discuss about the principle, requirements and procedure for staining of nucleic acid using silver nitrate. Principle: Silver salt is reduced to metallic silver which is then deposited in the immediate vicinity of the staining substratum and provides sensitive and reproducible stain. Requirement: Dissolve 60 g sodium carbonate in 2 liters […]
Staining of Nucleic Acids | Molecular Biology
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Polymerase Chain Reaction (PCR) | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Polymerase Chain Reaction (PCR). PCR is a simple method for in vitro enzymatic amplification of specific nucleic acids using multiple cycles of template denaturation, primer annealing and primer elongation. From as little as a single piece of target DNA, enough copies can […]