Upload NowIn this article we will discuss about the principle, requirements and procedure for purification of DNA. Principle: DNA is removed from gel by dissolving the gel in cesium chloride and DNA is taken out with ethidium bromide in T.E. buffer, centrifuged and DNA is removed. Ethidium bromide is extracted with n-butanol, and isopropanol precipitates out […]
Purification of DNA | Molecular Biology
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Random Amplified Polymorphic DNA (RAPD) Analysis | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Random Amplified Polymorphic DNA (RAPD) analysis. Randomly amplified polymorphic DNA (RAPD) was developed by William et al. (1990) to detect genetic polymorphism in crop plants. Polymerase chain reaction (PCR) is an in vitro method of nucleic acid synthesis by which a particular […]
Agarose Gel Electrophoresis | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Agarose gel electrophoresis. Agarose gel electrophoresis is a simple and highly effective method for separating, identifying and purifying DNA fragments. Separation is carried out under an electric field applied to gel matrix. Because of the negatively charged phosphates along the backbone, DNA […]