Upload NowIn this article we will discuss about the principle, requirements and procedure for staining of nucleic acid using silver nitrate. Principle: Silver salt is reduced to metallic silver which is then deposited in the immediate vicinity of the staining substratum and provides sensitive and reproducible stain. Requirement: Dissolve 60 g sodium carbonate in 2 liters […]
Staining of Nucleic Acids | Molecular Biology
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Southern Hybridisation | Molecular Biology
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Hybridisation reactions are based on the ability of denatured DNA to anneal to a complementary strand present in a nearby environment at a temperature below that of theirs. The two important features of hybridisation are: (1) These reactions are specific i.e. the probe will only bind to targets with complementary sequence. (2) These reactions will […]
Isolation of Bacterial Genomic DNA | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for isolation of bacterial genomic DNA. Bacterial chromosomes are single supercoiled double stranded circular DNA molecules. Plasmid DNA has a size of 0.1 to 0.5% of the chromosome. During plasmid purification preferential recovery of circular plasmid DNA over linear chromosomal (genomic) DNA is […]