Upload NowJ. Lederberg and E. Lederberg (1952) devised this procedure to demonstrate the spontaneous nature of mutations. This method is used for detection of biochemical mutants, for the classification of fermentation reactions and for the determination of the spectra of antibiotic sensitivity. Phage sensitive strain of E.coli on nutrient agar plate is incubated until each cell […]
Replica Plating: Principle and Procedure | Molecular Biology
Article shared by : 
Restriction Digestion of DNA and DNA Samples | Molecular Biology
Article shared by :
In this article we will discuss about the restriction digestion of DNA and DNA samples. Restriction Digestion of DNA: Restriction endonucleases cut DNA molecules into smaller pieces. They cut DNA at the specific recognition sequence, so that it is no longer susceptible to cleavage by the endonucleases. These enzymes are present in vivo in bacteria […]
Separating Micro-Weights of Proteins: PAGE | Molecular Biology
Article shared by :
In this article we will discuss about the principle, requirements and procedure for Polyacrylamide Gel Electrophoresis (PAGE). Micro-weights of proteins can be separated and characterised rapidly and relatively sensitive, using PAGE. Buffer gels (non-denaturing) as well as Sodium Dodecyl Sulphate (SDS) containing denaturing gels are used. Separation in buffer gels depends on both the charge […]