Upload NowIn this article we will discuss about the principle, requirements and procedure for isolation of plasmid DNA using alkaline lysis method. Principle: The alkaline lysis method of plasmid DNA is based on lysis of bacteria by treating it with SDS and NaOH, neutralisation of this mixture with potassium acetate which causes rapid annealing of the […]
Isolation of Plasmid DNA | Molecular Biology
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Replica Plating: Principle and Procedure | Molecular Biology
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J. Lederberg and E. Lederberg (1952) devised this procedure to demonstrate the spontaneous nature of mutations. This method is used for detection of biochemical mutants, for the classification of fermentation reactions and for the determination of the spectra of antibiotic sensitivity. Phage sensitive strain of E.coli on nutrient agar plate is incubated until each cell […]
Restriction Digestion of DNA and DNA Samples | Molecular Biology
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In this article we will discuss about the restriction digestion of DNA and DNA samples. Restriction Digestion of DNA: Restriction endonucleases cut DNA molecules into smaller pieces. They cut DNA at the specific recognition sequence, so that it is no longer susceptible to cleavage by the endonucleases. These enzymes are present in vivo in bacteria […]