Upload NowIn this article we will discuss about the principle, requirements and procedure for northern blotting and hybridisation. By this method specific mRNA sequences in RNA preparations can be detected. Northern blots are carried out in order to detect as to which of the several RNA molecules on agarose gel hybridize to a particular probe. It […]
Northern Blotting and Hybridisation | Molecular Biology
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Polymerase Chain Reaction (PCR) | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Polymerase Chain Reaction (PCR). PCR is a simple method for in vitro enzymatic amplification of specific nucleic acids using multiple cycles of template denaturation, primer annealing and primer elongation. From as little as a single piece of target DNA, enough copies can […]
Isolation of RNA | Molecular Biology
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In this article we will discuss about the requirements and procedure for isolation of RNA using Phenol-SDS method. SDS dissociates the ribonucleoprotein complex into RNA and protein, deproteinised by phenol and the free RNA left in aqueous solution is precipitated in cold after adding alcohol. Requirements: Reagents: 1. Extraction buffer-pH 9.0: Tris HCl (0.1 M): […]