Upload NowIn this article we will discuss about the principle, requirements and procedure for Amplified Fragment Length Polymorphism (AFLP) analysis. Amplified Fragment Length Polymorphism is a nucleic acid finger printing method to find out genetic variation that exists between closely related genes. This method was designed by Zabeau and Vos (1993) and Vos et al. (1995). […]
Amplified Fragment Length Polymorphism (AFLP) Analysis | Molecular Biology
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Silver Staining of Proteins | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for silver staining of proteins. Polypeptides even upto 0.1 µg present on gel can be visualised by staining with amido black 10B dye or Coomassie brilliant blue R250. Silver staining is useful since some proteins occur in very small quantities and are difficult […]
Staining of Nucleic Acids | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for staining of nucleic acid using silver nitrate. Principle: Silver salt is reduced to metallic silver which is then deposited in the immediate vicinity of the staining substratum and provides sensitive and reproducible stain. Requirement: Dissolve 60 g sodium carbonate in 2 liters […]