Upload NowIn this article we will discuss about the requirements and procedure for isolation of RNA using Phenol-SDS method. SDS dissociates the ribonucleoprotein complex into RNA and protein, deproteinised by phenol and the free RNA left in aqueous solution is precipitated in cold after adding alcohol. Requirements: Reagents: 1. Extraction buffer-pH 9.0: Tris HCl (0.1 M): […]
Isolation of RNA | Molecular Biology
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Purification of DNA | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for purification of DNA. Principle: DNA is removed from gel by dissolving the gel in cesium chloride and DNA is taken out with ethidium bromide in T.E. buffer, centrifuged and DNA is removed. Ethidium bromide is extracted with n-butanol, and isopropanol precipitates out […]
Random Amplified Polymorphic DNA (RAPD) Analysis | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Random Amplified Polymorphic DNA (RAPD) analysis. Randomly amplified polymorphic DNA (RAPD) was developed by William et al. (1990) to detect genetic polymorphism in crop plants. Polymerase chain reaction (PCR) is an in vitro method of nucleic acid synthesis by which a particular […]