Upload NowIn this article we will discuss about the principle, requirements and procedure for isolation of bacterial genomic DNA. Bacterial chromosomes are single supercoiled double stranded circular DNA molecules. Plasmid DNA has a size of 0.1 to 0.5% of the chromosome. During plasmid purification preferential recovery of circular plasmid DNA over linear chromosomal (genomic) DNA is […]
Isolation of Bacterial Genomic DNA | Molecular Biology
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Isolation of Plasmid DNA | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for isolation of plasmid DNA using alkaline lysis method. Principle: The alkaline lysis method of plasmid DNA is based on lysis of bacteria by treating it with SDS and NaOH, neutralisation of this mixture with potassium acetate which causes rapid annealing of the […]
Replica Plating: Principle and Procedure | Molecular Biology
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J. Lederberg and E. Lederberg (1952) devised this procedure to demonstrate the spontaneous nature of mutations. This method is used for detection of biochemical mutants, for the classification of fermentation reactions and for the determination of the spectra of antibiotic sensitivity. Phage sensitive strain of E.coli on nutrient agar plate is incubated until each cell […]