Upload NowIn this article we will discuss about the principle, requirements and procedure for silver staining of proteins. Polypeptides even upto 0.1 µg present on gel can be visualised by staining with amido black 10B dye or Coomassie brilliant blue R250. Silver staining is useful since some proteins occur in very small quantities and are difficult […]
Silver Staining of Proteins | Molecular Biology
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Staining of Nucleic Acids | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for staining of nucleic acid using silver nitrate. Principle: Silver salt is reduced to metallic silver which is then deposited in the immediate vicinity of the staining substratum and provides sensitive and reproducible stain. Requirement: Dissolve 60 g sodium carbonate in 2 liters […]
Southern Hybridisation | Molecular Biology
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Hybridisation reactions are based on the ability of denatured DNA to anneal to a complementary strand present in a nearby environment at a temperature below that of theirs. The two important features of hybridisation are: (1) These reactions are specific i.e. the probe will only bind to targets with complementary sequence. (2) These reactions will […]