Upload NowThe following points highlight the two methods used for extraction of plant genomic DNA. The methods are: 1. CTAB Method 2. Potassium Acetate Method. 1. CTAB Method: Plant genomic DNA is required mainly for mapping and isolation of genes for genetic engineering. If the protocol is good, pure and intact DNA can be obtained. The […]
Extraction of Plant Genomic DNA | Molecular Biology
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Replica Plating: Principle and Procedure | Molecular Biology
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J. Lederberg and E. Lederberg (1952) devised this procedure to demonstrate the spontaneous nature of mutations. This method is used for detection of biochemical mutants, for the classification of fermentation reactions and for the determination of the spectra of antibiotic sensitivity. Phage sensitive strain of E.coli on nutrient agar plate is incubated until each cell […]
Restriction Digestion of DNA and DNA Samples | Molecular Biology
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In this article we will discuss about the restriction digestion of DNA and DNA samples. Restriction Digestion of DNA: Restriction endonucleases cut DNA molecules into smaller pieces. They cut DNA at the specific recognition sequence, so that it is no longer susceptible to cleavage by the endonucleases. These enzymes are present in vivo in bacteria […]