Upload NowThis was the first type of transfer of genetic information discovered in bacteria. The first transformation was done by Fredrick Griffith in 1928. In this method a foreign plasmid DNA is introduced into a bacterium and uses this to multiply plasmids in order to obtain large amounts of it. Principle: The E. coli (DH5α) used […]
Transformation of Bacterial Gene | Molecular Biology
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Random Amplified Polymorphic DNA (RAPD) Analysis | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Random Amplified Polymorphic DNA (RAPD) analysis. Randomly amplified polymorphic DNA (RAPD) was developed by William et al. (1990) to detect genetic polymorphism in crop plants. Polymerase chain reaction (PCR) is an in vitro method of nucleic acid synthesis by which a particular […]
Agarose Gel Electrophoresis | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Agarose gel electrophoresis. Agarose gel electrophoresis is a simple and highly effective method for separating, identifying and purifying DNA fragments. Separation is carried out under an electric field applied to gel matrix. Because of the negatively charged phosphates along the backbone, DNA […]