Upload NowIn this article we will discuss about the technique of southern blotting for transfer of DNA from gels to nitrocellulose. This technique was first described by E.M. Southern in 1975. It is useful for analysis of Restriction Fragment Length Polymorphisms (RFLPs). Using gel electrophoresis in combination with restriction endonuclease analysis, a detailed and accurate physical […]
Technique of Southern Blotting | DNA | Molecular Biology
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Isolation of RNA | Molecular Biology
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In this article we will discuss about the requirements and procedure for isolation of RNA using Phenol-SDS method. SDS dissociates the ribonucleoprotein complex into RNA and protein, deproteinised by phenol and the free RNA left in aqueous solution is precipitated in cold after adding alcohol. Requirements: Reagents: 1. Extraction buffer-pH 9.0: Tris HCl (0.1 M): […]
Purification of DNA | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for purification of DNA. Principle: DNA is removed from gel by dissolving the gel in cesium chloride and DNA is taken out with ethidium bromide in T.E. buffer, centrifuged and DNA is removed. Ethidium bromide is extracted with n-butanol, and isopropanol precipitates out […]