Upload NowThe following points highlight the two methods used for extraction of plant genomic DNA. The methods are: 1. CTAB Method 2. Potassium Acetate Method. 1. CTAB Method: Plant genomic DNA is required mainly for mapping and isolation of genes for genetic engineering. If the protocol is good, pure and intact DNA can be obtained. The […]
Extraction of Plant Genomic DNA | Molecular Biology
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Northern Blotting and Hybridisation | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for northern blotting and hybridisation. By this method specific mRNA sequences in RNA preparations can be detected. Northern blots are carried out in order to detect as to which of the several RNA molecules on agarose gel hybridize to a particular probe. It […]
Polymerase Chain Reaction (PCR) | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Polymerase Chain Reaction (PCR). PCR is a simple method for in vitro enzymatic amplification of specific nucleic acids using multiple cycles of template denaturation, primer annealing and primer elongation. From as little as a single piece of target DNA, enough copies can […]