Upload NowIn gene cloning, the major steps are cutting and joining lengths of DNA using restriction endonuclease and ligase. Several steps are involved in gene cloning. 1. Preparation of cloning vector: Before cloning the fragment of interest, the plasmid (cloning vector) is to be restriction digested. Plasmids are small extra chromosomal, self-replicating, circular double stranded DNA […]
Steps in Gene Cloning (With Experiment) | Molecular Biology
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Transformation of Bacterial Gene | Molecular Biology
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This was the first type of transfer of genetic information discovered in bacteria. The first transformation was done by Fredrick Griffith in 1928. In this method a foreign plasmid DNA is introduced into a bacterium and uses this to multiply plasmids in order to obtain large amounts of it. Principle: The E. coli (DH5α) used […]
Immunoadsorption: Principle and Procedure | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for immunoadsorption. Principle: Antigen-antibody complexes are formed when transferred proteins on nitrocellulose paper are allowed to react with diluted antis- era. This antigen-antibody complex bound to the nitrocellulose membrane are allowed to react with alkaline phosphatase conjugated anti-rabbit-IgG. The colour develops blue to […]