Upload NowIn this article we will discuss about the rules, precautions and basic requirements of a laboratory. Laboratory Rules and Precautions: A new worker in a laboratory must have an orientation before starting the work. The new worker should be given instructions as to how to operate major equipment including microscope, analytical balance and pH meter. […]
Laboratory: Rules and Basic Requirements | Biotechnology
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Capillary Blotting: Principle and Procedure | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for capillary blotting. Principle: Samples of proteins isolated on gel are transported out of gel by capillary action of buffer. The solubility of proteins increases by the presence of SDS and helps its migration to nitrocellulose membrane which binds it strongly on its […]
Technique of Southern Blotting | DNA | Molecular Biology
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In this article we will discuss about the technique of southern blotting for transfer of DNA from gels to nitrocellulose. This technique was first described by E.M. Southern in 1975. It is useful for analysis of Restriction Fragment Length Polymorphisms (RFLPs). Using gel electrophoresis in combination with restriction endonuclease analysis, a detailed and accurate physical […]