In this article we will discuss about the principle, requirements and procedure for isolation of coliphages from sewage. Principle: Samples like raw sewage when inoculated with a susceptible host on an agar medium will form plaques of the phage. There are various steps involved in isolation of phages: (i) Collection of phage containing samples from […]
Isolation of Coliphages from Sewage | Microbial Biotechnology
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Litmus Milk Reactions: Requirements and Procedure | Bacteria
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After reading this article you will learn about the requirements and procedure of litmus milk reactions. Milk sugar lactose, the milk proteins casein, lacto-albumin and lacto-globulin are the milk substrates that can be transformed by microorganisms, bringing about changes in pH. In order to distinguish the metabolic changes, a pH indicator, the oxidation reduction indicator […]
Isolation of Bacteria in Soil | Microbial Biotechnology
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In this article we will discuss about the isolation of bacteria present in soil:- 1. Methods of Isolation 2. Observation of Bacteria 3. Staining of Bacteria 4. Staining Procedure 5. Staining of Spore 6. Staining of Capsule 7. Staining of Flagella. Methods of Isolation: Bacteria present in soil can be isolated by several methods. However, […]