Upload NowThe following points highlight the top two methods used for cultivation of anaerobic organisms. The methods are: 1. Shake Culture Method 2. Pyrogallic Acid: NaOH Method. 1. Shake Culture Method: Principle: The anaerobic organism does not require molecular oxygen for growth and hence will grow within the agar medium and E. coli above the medium […]
Cultivation of Anaerobic Organisms | Microbial Biotechnology
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Actinomycetes: Isolation and Identification | Microbial Biotechnology
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In this article we will discuss about the isolation and identification of actinomycetes. Isolation of Actinomycetes: This group of prokaryotes are ubiquitious and are found in all ecosystems. Some genera like Actinomyces are anaerobic and others like Frankia (symbiotic) require specialised growth media and incubation condition. To isolate them from soil, special treatments are essential. […]
Isolation of Bacteria in Soil | Microbial Biotechnology
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In this article we will discuss about the isolation of bacteria present in soil:- 1. Methods of Isolation 2. Observation of Bacteria 3. Staining of Bacteria 4. Staining Procedure 5. Staining of Spore 6. Staining of Capsule 7. Staining of Flagella. Methods of Isolation: Bacteria present in soil can be isolated by several methods. However, […]