After reading this article you will learn about the requirements and procedure of litmus milk reactions. Milk sugar lactose, the milk proteins casein, lacto-albumin and lacto-globulin are the milk substrates that can be transformed by microorganisms, bringing about changes in pH. In order to distinguish the metabolic changes, a pH indicator, the oxidation reduction indicator […]
Litmus Milk Reactions: Requirements and Procedure | Bacteria
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Cultivation of Anaerobic Organisms | Microbial Biotechnology
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The following points highlight the top two methods used for cultivation of anaerobic organisms. The methods are: 1. Shake Culture Method 2. Pyrogallic Acid: NaOH Method. 1. Shake Culture Method: Principle: The anaerobic organism does not require molecular oxygen for growth and hence will grow within the agar medium and E. coli above the medium […]
Actinomycetes: Isolation and Identification | Microbial Biotechnology
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In this article we will discuss about the isolation and identification of actinomycetes. Isolation of Actinomycetes: This group of prokaryotes are ubiquitious and are found in all ecosystems. Some genera like Actinomyces are anaerobic and others like Frankia (symbiotic) require specialised growth media and incubation condition. To isolate them from soil, special treatments are essential. […]