Upload NowThe following points highlight the two main tests conducted for utilisation of amino acids:- 1. Decarboxylase Test 2. Phenylalanine Deaminase Test. 1. Decarboxylase Test: This test helps in differentiating Gram-negative organisms based on their ability to degrade amino acid substrates enzymatically. All biologically active proteins are composed of 20 essential amino acids. Amino acids have […]
Utilisation of Amino Acids | Microbial Biotechnology
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Rhizobium: Isolation and Authentication | Microbial Biotechnology
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In this article we will discuss about the isolation and authentication of rhizobium. Isolation of Rhizobium: Rhizobium is a symbiotic N2 fixer found to occur as bacteroids in the root nodules of leguminous plants. They can be easily isolated and cultured in vitro. Rhizobia are Gram- negative rods which are motile with bi-polar, sub-polar and […]
Measuring the Growth of Bacteria | Microbial Biotechnology
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In this article we will discuss about the principle, requirements and procedure for measuring the growth of bacteria by spectrophotometric method. Principle: This method relates cell number to turbidity of a broth culture. Turbidity or optical density (OD) is directly proportional to cell concentration. Requirements: 1. 10-12 hour E. coli broth culture maintained at lag […]