Tag Archives | Isolation of RNA

In this article we will discuss about the requirements and procedure for isolation of RNA using Phenol-SDS method. SDS dissociates the ribonucleoprotein com­plex into RNA and protein, deproteinised by phenol and the free RNA left in aqueous so­lution is precipitated in cold after adding alco­hol. Requirements: Reagents: 1. Extraction buffer-pH 9.0: Tris HCl (0.1 M): […]