Upload NowIn this article we will discuss about the pH meter and its operation. pH meter is a potentiometer that measures the voltage between two electrodes dipped in a solution. One of these electrodes is a calomel electrode and the other a glass electrode. The calomel electrode has a constant electrical potential and is the external […]
pH Meter and Its Operation | Laboratory | Biotechnology
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Spectrophotometer: Meaning, Parts and Operation | Biotechnology
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In this article we will discuss about:- 1. Meaning of Spectrophotometer 2. Parts of Spectrophotometer 3. Operation. Meaning of Spectrophotometer: Spectrophotometer measures light absorption as a function of wavelength in UV as well as visible regions and follows the Beer Lambert’s law of light absorption. Beer Lambert’s Law: When a monochromatic light passes through an […]
Determination of pKa Values using pH Meter | Biotechnology
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In this article we will discuss about the determination of pKa values using pH meter. Titration between a Strong Acid and a Strong Base: Requirements: 1. pH meter. 2. 0.1(M) HCl (Normality of HCl is ll.5). 3. 0.1 (M) NaOH (dilute from 1(M) stock solution). 4. Standard buffer 0.05(M) potassium hydrogen phthalate, pH 4.01. 5. […]