In this article we will discuss about the requirements, reagents and procedure for extraction and separation of storage proteins. Requirements: 1. Legume seeds/serial grains. 2. Grinder. 3. Refrigerator. 4. Centrifuge. 5. Spectronic 20. 6. Magnetic stirrer. 7. TRIS base. 8. NaCl. 9. Anhydrous Na2SO4. 10. NaOH. 11. Na-K-tartarate. 12. Na2CO3. 13. CuSO4.7H2O. 14. Folin-Ciocalteau reagent. […]