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Estimation of RNA | Biotechnology

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In this article we will discuss about the principle, requirements and procedure for estimation of RNA. Principle: Ribose (a pentose) in presence of hot acid is converted to furfural which then reacts with orcinol to yield a green colour, read at 660 nm. Requirements: 1. Orcinol: Prepare 10% (W/V) FeCl3. 6H20 solution. To 0.5 ml […]

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Tag Archives | Estimation of RNA