Upload NowThe following points highlight the top three methods used for estimation of DNA. The methods are: 1. Witham et. al.( 1971) 2. Ethidium Bromide Method 3. U V Spectrophotometric Method. 1. Method by Witham et. al.( 1971): Principle: DNA is initially depurinated quantitatively, followed by the dehydration of sugar to hydroxylevulinylaldehyde. In an acidic medium, […]
Estimation of DNA: 3 Methods | Biotechnology
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Gene Isolation and Cloning of DNA | Biotechnology
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In this article we will discuss about the gene isolation and cloning of DNA. The formation of new combinations of genetic material by the insertion of nucleic acid produced outside the cell into a virus, bacterial plasmid or any other vector system to allow its incorporation into a host organism in which it is capable […]
6 Methods of DNA Sequencing | Biotechnology
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The following are the common methods of DNA sequencing:- 1. Sequencing 2. Manual Sequencing 3. Automated Sequencing 4. Southern Blotting 5. Northern Blotting 6. Western Blotting. Method # 1. Sequencing: One of the major methods of DNA sequencing in known as chain termination sequencing, dideoxy sequencing, or Sanger sequencing after its inventor biochemist Frederick Sanger. […]