In this article we will discuss about DNA isolation with its protocol. Learn how to isolate:- 1. Plasmid DNA 2. Bacterial Genomic DNA 3. Yeast Genomic DNA 4. Genomic DNA from Blood 5. DNA from Animal Cells 6. Genomic DNA from Eukaryotic Tissues 7. Plant DNA using CTAB Extraction Method 8. Chloroplast DNA 9. Mitochondrial […]
How to Isolate DNA? (With Protocols) | Biotechnology
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Blotting of DNA: Southern, Northern and Western | Biotechnology
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In this article we will discuss about the southern, northern and western blotting of DNA. Southern Blotting of DNA: DNA fragments on agarose gels give no indication of function or sequence. Blotting is a technique used to identify specific DNA sequences. Usually, larger fragments of DNA (genomic DNA) are made into conveniently sized fragments by […]
Technique of Southern Blotting | DNA | Molecular Biology
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In this article we will discuss about the technique of southern blotting for transfer of DNA from gels to nitrocellulose. This technique was first described by E.M. Southern in 1975. It is useful for analysis of Restriction Fragment Length Polymorphisms (RFLPs). Using gel electrophoresis in combination with restriction endonuclease analysis, a detailed and accurate physical […]