Upload NowIn this article we will discuss about the principle, requirements and procedure for estimation of total free amino acids that exist in plant and animal tissues. Amino acids exist free as well as bound form as proteins in plant and animal tissues. They are colourless ionic compounds that form the building blocks of proteins. Most […]
Estimation of Total Free Amino Acids | Biotechnology
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Estimation of DNA: 3 Methods | Biotechnology
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The following points highlight the top three methods used for estimation of DNA. The methods are: 1. Witham et. al.( 1971) 2. Ethidium Bromide Method 3. U V Spectrophotometric Method. 1. Method by Witham et. al.( 1971): Principle: DNA is initially depurinated quantitatively, followed by the dehydration of sugar to hydroxylevulinylaldehyde. In an acidic medium, […]
Estimation of Enzymes | Biotechnology
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In this article we will discuss about the estimation of various enzymes:- 1. Catalase 2. Peroxidase (Pod) 3. Polyphenol Oxidase (PPO) 4. Indol Acetic Acid Oxidase 5. Xylanase 6. Cellulases 7. Lipase 8. Nitrate Reductase 9. Nitrite Reductase 10. Aflatoxin 11. Phenolics 12. Ergot Alkaloids. Catalase: This enzyme catalyses (1) Decomposition of H2O2 to give […]