Upload NowIn this article we will discuss about the principle, requirements and procedure for estimation of RNA. Principle: Ribose (a pentose) in presence of hot acid is converted to furfural which then reacts with orcinol to yield a green colour, read at 660 nm. Requirements: 1. Orcinol: Prepare 10% (W/V) FeCl3. 6H20 solution. To 0.5 ml […]
Estimation of RNA | Biotechnology
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Estimation of Total Free Amino Acids | Biotechnology
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In this article we will discuss about the principle, requirements and procedure for estimation of total free amino acids that exist in plant and animal tissues. Amino acids exist free as well as bound form as proteins in plant and animal tissues. They are colourless ionic compounds that form the building blocks of proteins. Most […]
Estimation of DNA: 3 Methods | Biotechnology
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The following points highlight the top three methods used for estimation of DNA. The methods are: 1. Witham et. al.( 1971) 2. Ethidium Bromide Method 3. U V Spectrophotometric Method. 1. Method by Witham et. al.( 1971): Principle: DNA is initially depurinated quantitatively, followed by the dehydration of sugar to hydroxylevulinylaldehyde. In an acidic medium, […]