In this article we will discuss about the principle, requirements and procedure for estimation of nitrogenase. Nitrogenase is possessed by a group of prokaryotes which breaks the triple bond of elemental nitrogen (N≡N) present in the atmosphere and convert it into NH3, an assimilable form for plants. This enzyme can also reduce acetylene to ethylene. […]
Estimation of Nitrogenase | Enzymes| Biotechnology
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Inoculum Production of Nitrogen Fixers | Biotechnology
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For the preparation of this inoculant there are several criteria to be followed, e.g. strain selections. For Rhizobium, strains that are compatible and produce effective nodules should be selected. Individual legumes have specific strains and hence it is difficult to prepare inoculum with all strains since one strain is likely to dominate over the others. […]
Biochemical Oxygen Demand (BOD) in Water | Biotechnology
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In this article we will discuss about the principle, requirements and procedure for determination of Biochemical Oxygen Demand (BOD) in water. Principle: The sewage/water is incubated at 20°C in BOD incubator for 5 days and the amount of dissolved oxygen is calculated along with blanks and both initial and final volume of O2 is calculated. […]