In this article we will discuss about the determination of pKa values using pH meter. Titration between a Strong Acid and a Strong Base: Requirements: 1. pH meter. 2. 0.1(M) HCl (Normality of HCl is ll.5). 3. 0.1 (M) NaOH (dilute from 1(M) stock solution). 4. Standard buffer 0.05(M) potassium hydrogen phthalate, pH 4.01. 5. […]
Determination of pKa Values using pH Meter | Biotechnology
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Isolation of Azotobacter Species from Soil | Biotechnology
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In this article we will discuss about the requirements and procedure for isolation of azotobacter species from soil. Azotobacter is a free-living N2 fixer found in soil. They have relatively large size when observed under phase contrast microscope. They are peritrichous or has polar flagella and form thick-walled microcysts in some species. They are obligate […]
Isolation and Identification of Fungi | Biotechnology
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In this article we will discuss about the isolation and identification of fungi. Isolation of Fungi: 1. Dilution Plate Method. 2. Soil Plate Method. This method is used to study the ecological distribution of various soil fungi. Principle: In this method no fungal spore will be lost as in that of soil dilution and gives […]