Upload NowIn this article we will discuss about the determination of pKa values using pH meter. Titration between a Strong Acid and a Strong Base: Requirements: 1. pH meter. 2. 0.1(M) HCl (Normality of HCl is ll.5). 3. 0.1 (M) NaOH (dilute from 1(M) stock solution). 4. Standard buffer 0.05(M) potassium hydrogen phthalate, pH 4.01. 5. […]
Determination of pKa Values using pH Meter | Biotechnology
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Laboratory: Rules and Basic Requirements | Biotechnology
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In this article we will discuss about the rules, precautions and basic requirements of a laboratory. Laboratory Rules and Precautions: A new worker in a laboratory must have an orientation before starting the work. The new worker should be given instructions as to how to operate major equipment including microscope, analytical balance and pH meter. […]
Estimation of Leghaemoglobin | Leguminous Plants | Biotechnology
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In this article we will discuss about the principle, requirements and procedure for estimation of leghaemoglobin. Kubo (1939) recognised the oxygen-binding haemoprotein in the root nodules of leguminous plants. Hence pan of the Leghaemoglobin is synthesised by the bacterium (Rhizobium) and the apoprotein (globin) is coded by plant DNA Oxygen is diffused by Lb across […]