In this article we will discuss about the estimation and micro estimation of citric acid. Estimation of Citric Acid: Titrable Acidity Method: Requirements: 1. Fermented broth. 2. 0.1 (N) NaOH. 3. 1.0(N) Succinic acid. 4. Phenolphthalin. 5. Titration set. Procedure: 1. Standardise NaOH using 1(N) succinic acid. 2. Take in two separate 100 ml conical […]
Estimation and Micro Estimation of Citric Acid | Biotechnology
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Isolation of Plant Pathogens | Biotechnology
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In this article we will discuss about the principle, requirements and procedure for isolation of plant pathogens. Principle: A medium with a carbon source and inorganic salts will permit the growth of plant pathogens which can be maintained on agar slants of the same medium. Requirements: 1. Infected plant parts, leaves/stem infected with Alternaria. 2. […]
Isolation of Fungi | Biotechnology
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In this article we will discuss about the isolation of specific groups of fungi: 1. Acrasiales 2. Chytridiales 3. Saprolegniales and Pythiales 4. Ascomycotina 5. Yeasts. 1. Acrasiales: The food used by this group of organisms is mainly bacteria. Requirements: a. Soil sample. b. Escherichia coli cultures. c. Nutrient agar plates. d. 25 ml specimen […]