In this article we will discuss about:- 1. Isolation of VAM Spores from Soil 2. Measurement of Root Infection 3. Most Probable Number (MPN) 4. Identification of VAM Spores 5. Pure Inoculum Production 6. Mass Production 7. Pot Cultures 8. Aeroponic Cultures. Endotrophic mycorrhiza or vesicular arbuscular mycorrhizae are known so, since they produce vesicles […]
Vesicular Arbuscular Mycorrhiza (VAM) | Biotechnology
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Screening of Antibiotic Producing Organisms | Biotechnology
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The following points highlight the top two techniques used for screening of antibiotic producing organisms. The techniques are: 1. Crowded Plate Technique 2. Determination of Antimicrobial Spectrum Giant Colony Technique. 1. Crowded Plate Technique: Principle: When soil or suspension with lower dilutions is inoculated, large number of microorganisms grow on the agar plate. Those which […]
Rhizobium: Isolation and Authentication | Microbial Biotechnology
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In this article we will discuss about the isolation and authentication of rhizobium. Isolation of Rhizobium: Rhizobium is a symbiotic N2 fixer found to occur as bacteroids in the root nodules of leguminous plants. They can be easily isolated and cultured in vitro. Rhizobia are Gram- negative rods which are motile with bi-polar, sub-polar and […]