Upload NowIn this article we will discuss about:- 1. Meaning of Spectrophotometer 2. Parts of Spectrophotometer 3. Operation. Meaning of Spectrophotometer: Spectrophotometer measures light absorption as a function of wavelength in UV as well as visible regions and follows the Beer Lambert’s law of light absorption. Beer Lambert’s Law: When a monochromatic light passes through an […]
Spectrophotometer: Meaning, Parts and Operation | Biotechnology
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Methods of Sterilisation | Laboratory | Biotechnology
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The following points highlight the top eight methods of sterilization. The methods are: 1. Boiling 2. Intermittent Heating 3. Autoclaving 4. Dry Heat Sterilisation Indirect Heat 5. Open Flame Sterilisation 6. Filtration 7. Chemical Sterilisation 8. Pasteurisation. Method # 1. Boiling: In the absence of heat resistant spores, boiling will free liquids of living cells. […]
Preparation of Buffers and Solutions | Laboratory | Biotechnology
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The following guidelines should be followed while making reagents, buffers, etc. 1. Use highest grade of reagents wherever possible. 2. Prepare all solutions with double distilled or distilled water. 3. Autoclave solutions whenever possible. Solutions that cannot withstand autoclaving should be sterilized using 0.2 mm filter. 4. pH meter should carefully be checked using freshly […]