In this article we will discuss about the fermentation of penicillin. Requirements: 1. Starter cultures on M2 Agar. 2. M2 agar (pH 7.0): 3. Seed medium: 4. Fermentation medium: Dissolve 1.5 g of phenyl acetic acid in warm distilled water. Adjust pH to 7 and make up the volume to 100 ml. Add 1 […]
Fermentation of Penicillin | Antibiotics | Biotechnology
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Spectrophotometer: Meaning, Parts and Operation | Biotechnology
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In this article we will discuss about:- 1. Meaning of Spectrophotometer 2. Parts of Spectrophotometer 3. Operation. Meaning of Spectrophotometer: Spectrophotometer measures light absorption as a function of wavelength in UV as well as visible regions and follows the Beer Lambert’s law of light absorption. Beer Lambert’s Law: When a monochromatic light passes through an […]
Methods of Sterilisation | Laboratory | Biotechnology
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The following points highlight the top eight methods of sterilization. The methods are: 1. Boiling 2. Intermittent Heating 3. Autoclaving 4. Dry Heat Sterilisation Indirect Heat 5. Open Flame Sterilisation 6. Filtration 7. Chemical Sterilisation 8. Pasteurisation. Method # 1. Boiling: In the absence of heat resistant spores, boiling will free liquids of living cells. […]