In this article we will discuss about the pH meter and its operation. pH meter is a potentiometer that measures the voltage between two electrodes dipped in a solution. One of these electrodes is a calomel electrode and the other a glass electrode. The calomel electrode has a constant electrical potential and is the external […]
pH Meter and Its Operation | Laboratory | Biotechnology
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Screening of Antibiotic Producing Organisms | Biotechnology
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The following points highlight the top two techniques used for screening of antibiotic producing organisms. The techniques are: 1. Crowded Plate Technique 2. Determination of Antimicrobial Spectrum Giant Colony Technique. 1. Crowded Plate Technique: Principle: When soil or suspension with lower dilutions is inoculated, large number of microorganisms grow on the agar plate. Those which […]
Rhizobium: Isolation and Authentication | Microbial Biotechnology
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In this article we will discuss about the isolation and authentication of rhizobium. Isolation of Rhizobium: Rhizobium is a symbiotic N2 fixer found to occur as bacteroids in the root nodules of leguminous plants. They can be easily isolated and cultured in vitro. Rhizobia are Gram- negative rods which are motile with bi-polar, sub-polar and […]