In this article we will discuss about the setting up of a tissue culture laboratory that needs proper planning and depends upon space availability, volume of work to be carried out and funds. The available laboratory space is divided into two distinct laboratory areas i.e., media preparation area and aseptic technique. 1. Media Preparation Area: […]
Setting up of a Tissue Culture Laboratory | Biotechnology
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Isolation of Animal Tissue Culture | Biotechnology
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In this article we will discuss about the isolation of animal tissue culture. Before culture, animal materials must be washed in BSS (balanced salt solution) aseptically to avoid contamination. The tissue to be cultured should be properly sterilised with 70% ethanol, and remove surgically under aseptic conditions. Thus the tissue isolated should be either stored […]
6 Methods of DNA Sequencing | Biotechnology
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The following are the common methods of DNA sequencing:- 1. Sequencing 2. Manual Sequencing 3. Automated Sequencing 4. Southern Blotting 5. Northern Blotting 6. Western Blotting. Method # 1. Sequencing: One of the major methods of DNA sequencing in known as chain termination sequencing, dideoxy sequencing, or Sanger sequencing after its inventor biochemist Frederick Sanger. […]