In this article we will discuss about the principle, requirements and procedure for measuring the growth of bacteria by spectrophotometric method. Principle: This method relates cell number to turbidity of a broth culture. Turbidity or optical density (OD) is directly proportional to cell concentration. Requirements: 1. 10-12 hour E. coli broth culture maintained at lag […]
Measuring the Growth of Bacteria | Microbial Biotechnology
Isolation of Frankia | Bacteria| Microbial Biotechnology
In this article we will discuss about the requirements and procedure for isolation of frankia. Frankia is an actinomycete which forms symbiotic association with non-leguminous plants and fix atmospheric nitrogen. There are over 24 genera from eight angiosperm families which have been reported to possess actinorhizal nodules on the root system. Abortive attempts to isolate […]
Litmus Milk Reactions: Requirements and Procedure | Bacteria
After reading this article you will learn about the requirements and procedure of litmus milk reactions. Milk sugar lactose, the milk proteins casein, lacto-albumin and lacto-globulin are the milk substrates that can be transformed by microorganisms, bringing about changes in pH. In order to distinguish the metabolic changes, a pH indicator, the oxidation reduction indicator […]